Iron

For the iron chemistry project, seawater samples are collected during our annual relief voyages to Antarctica on board RV Agulhas II. A GEOTRACES trace metal clean 24 x 12L GoFlo carousel and Kevlar cable winch (Figure 1) is used to collect seawater along a vertical profile. Subsampling is conducted inside a certified trace metal clean container lab equipped with circulating HEPA filters for clean air supply (Figures 2 and 3). Concentrations for total dissolvable Fe (TDFe, unfiltered), dissolved Fe (DFe, filtered through 0.22 µm) and soluble Fe (SFe, filtered through 0.02µm) are measured using a Flow Injection Analyser (FIA; Figures 3 and 4) with chemiluminescence detection inside a trace metal clean class 100 laboratory at Stellenbosch University. An ICP-MS is also available at Stellenbosch University.

Seawater samples are also collected from the GoFlo bottles for the incubation experiments (Figure 6). Phytoplankton physiological parameters are measured using a Chelsea Fast Repetition fluorimeter (FRRf; Figure 4), dissolved Fe using the dedicated FIA in the trace metal clean laboratory at Stellenbosch University, and macro-nutrients using FIA at CSIR. The culture experiments are also conducted inside the trace metal clean class 1000 laboratory at Stellenbosch University

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For the iron chemistry project, seawater samples are collected during our annual relief voyages to Antarctica on board RV Agulhas II. A GEOTRACES trace metal clean 24 x 12L GoFlo carousel and Kevlar cable winch (Figure 1) is used to collect seawater along a vertical profile. Subsampling is conducted inside a certified trace metal clean container lab equipped with circulating HEPA filters for clean air supply (Figures 2 and 3). Concentrations for total dissolvable Fe (TDFe, unfiltered), dissolved Fe (DFe, filtered through 0.22 µm) and soluble Fe (SFe, filtered through 0.02µm) are measured using a Flow Injection Analyser (FIA; Figures 3 and 4) with chemiluminescence detection inside a trace metal clean class 100 laboratory at Stellenbosch University. An ICP-MS is also available at Stellenbosch University.

Seawater samples are also collected from the GoFlo bottles for the incubation experiments (Figure 6). Phytoplankton physiological parameters are measured using a Chelsea Fast Repetition fluorimeter (FRRf; Figure 4), dissolved Fe using the dedicated FIA in the trace metal clean laboratory at Stellenbosch University, and macro-nutrients using FIA at CSIR. The culture experiments are also conducted inside the trace metal clean class 1000 laboratory at Stellenbosch University